7/24/2023 0 Comments Bacterial blight of pomegranatePathogenicity of the representative bacterial strain Serik-4 was performed on 2-year-old pomegranate plants cv. Bacterial strains were characterized as gram negative, oxidase negative, catalase positive, tobacco hypersensitivity positive, and able to produce acid from L-arabinose, D-galactose, D-glucose, and D-mannitol but not from D-xylose. Bright yellow bacterial colonies were consistently isolated. The pathogen was isolated from leaf spots on naturally infected plants showing typical symptoms onto yeast dextrose chalk agar. Symptoms were characterized by dark brown, angular to irregularly shaped spots on leaves and fruit cankers on stems, branches, and trunks and split trunks. Typical bacterial blight symptoms were observed since spring of 2011 in pomegranate orchards located in Antalya Province. Īmruta N, Kumar MKP, Kandikattu HK et al (2019) Bio-priming of rice seeds with novel bacterial strains, for management of seedborne Magnaporthe oryzae L.Pomegranate (Punica granatum L.) is an increasingly important fruit crop that is widely cultivated in Turkey. J Pure Appl Microbiol 10:1043–1054Īmruta N, Prasanna Kumar MK, Puneeth ME et al (2018) Exploring the potentiality of novel rhizospheric bacterial strains against the rice blast fungus Magnaporthe oryzae. Īmruta N, Kumar MP, Narayanaswamy S et al (2016) Isolation and identification of rice blast disease-suppressing antagonistic bacterial strains from the rhizosphere of rice. Ībdelmoteleb A, Troncoso-Rojas R, Gonzalez-Soto T, González-Mendoza D (2017) Antifungical activity of autochthonous Bacillus subtilis isolated from prosopis juliflora against phytopathogenic fungi. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.ĭeutsch EW, Csordas A, Sun Z et al (2016) The proteomexchange consortium in 2017: supporting the cultural change in proteomics public data deposition. velezensis can be employed for controlling the bacterial and fungal pathogens of crop plants.īacillus velezensis Biocontrol Magnaporthe oryzae Secondary metabolites Whole-cell protein profiling Xanthomonas axonopodis pv. From the results obtained, the crude secondary metabolites and cell-free filtrates (containing bio-macromolecules) of the strains A6 and P42 of B. punicae, which eventually resulted in a higher inhibition zone of 3 cm, whereas the cell-free extract (75%) of the same strain significantly suppressed the growth of the pathogen with an inhibition zone of 1.48 cm. Similarly, in vitro evaluation of crude extract (10%) of P42 strain inhibited bacterial blight of pomegranate pathogen, Xanthomonas axonopodis pv. The in vitro evaluation of crude extracts (10%) of A6 and P42 significantly inhibited the rice pathogen, Magnaporthe oryzae (MG01), whereas the cell-free culture filtrate (75%) of strain P42 showed 58.97% inhibition. velezensis by nano-ESI LC-MS/MS revealed the presence of 9 proteins, respectively. Whole-cell protein profiling of A6 and P42 strains of B. Gas Chromatography analysis revealed that the A6 and P42 strains exert different functional groups of compounds, such as aromatic ring, aliphatic, alkene, ketone, amine groups and carboxylic acid. The bioactive compounds, specifically secondary metabolites, were characterized by whole-cell protein profiling, Thin-Layer Chromatography, Infra-Red Spectroscopy, Nuclear Magnetic Resonance, Gas Chromatograph and Electro Spray Liquid Chromatography. In the present study, we aimed to investigate the bio-macromolecules, such as proteins and peptides of Bacillus velezensis strains, namely A6 and P42 by whole-cell sequencing and highlighted the potential application in controlling phytopathogens. Bacillus velezensis is widely known for its inherent biosynthetic potential to produce a wide range of bio-macromolecules and secondary metabolites, including polyketides (PKs) and siderophores, as well as ribosomally and non-ribosomally synthesized peptides.
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